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Major Publications


100,000 Genomes Pilot on Rare-Disease Diagnosis in Health Care - Preliminary Report.

100,000 Genomes Project Pilot Investigators,[...], Floto RA, et al.

N Engl J Med. 2021 Nov 11;385(20):1868-1880. 

PMID: 34758253

The removal of airborne SARS-CoV-2 and other microbial bioaerosols by air filtration on COVID-19 surge units.

Conway Morris A, [...], Floto RA, et al.

Clin Infect Dis. 2021 Oct 30:ciab933.

PMID: 34718446

RNA-sequencing elucidates drug-specific mechanisms of antibiotic tolerance and resistance in M. abscessus.

Schildkraut JA, Coolen JPM, Burbaud S, Sangen JJN, Kwint MP, Floto RA, et al.

Antimicrob Agents Chemother. 2021 Oct 11:AAC0150921.

PMID: 34633851

***Dissemination of Mycobacterium abscessus via global transmission networks.

Ruis C, Bryant JM, Bell SC, Thomson R, Davidson RM, Hasan NA, van Ingen J, Strong M, Floto RA, Parkhill J.

Nat Microbiol. 2021 Oct;6(10):1279-1288. 

PMID: 34545208

***Integrated human/SARS-CoV-2 metabolic models present novel treatment strategies against COVID-19.

Bannerman BP, Júlvez J, Oarga A, Blundell TL, Moreno P, Floto RA.

Life Sci Alliance. 2021 Aug 5;4(10):e202000954. 

PMID: 34353886

Increased Virulence of Outer Membrane Porin Mutants of Mycobacterium abscessus.

de Moura VCN, Verma D, Everall I, Brown KP, Belardinelli JM, Shanley C, Stapleton M, Parkhill J, Floto RA, Ordway DJ, Jackson M.

Front Microbiol. 2021 Jul 14;12:706207. doi: 10.3389/fmicb.2021.706207. 

PMID: 34335541

A paradigm shift to combat indoor respiratory infection.

Morawska L, [...], Floto A, et al.,

Science. 2021 May 14;372(6543):689-691. doi: 10.1126/science.abg2025.

PMID: 33986171

*** Stepwise pathogenic evolution of Mycobacterium abscessus.

Bryant JM, Brown KP, Burbaud S, Everall I, Belardinelli JM, Rodriguez-Rincon D, Grogono DM, Peterson CM, Verma D, Evans IE, Ruis C, Weimann A, Arora D, Malhotra S, Bannerman B, Passemar C, Templeton K, MacGregor G, Jiwa K, Fisher AJ, Blundell TL, Ordway DJ, Jackson M, Parkhill J, Floto RA.

Science. 2021 Apr 30;372(6541):eabb8699. doi: 10.1126/science.abb8699.

PMID: 33926925

The Impact of Hospital-Ward Ventilation on Airborne-Pathogen Exposure.

Mingotti N, Grogono D, [...], Floto RA, Woods AW.

Am J Respir Crit Care Med. 2021 Mar 15;203(6):766-769. doi: 10.1164/rccm.202009-3634LE.

PMID: 33211973 

A multicentre analysis of Clostridium difficile in persons with Cystic Fibrosis demonstrates that carriage may be transient and highly variable with respect to strain and level.

Deane J, [...], Floto A, Rea MC, Ross RP, Stanton C, Plant BJ.

J Infect. 2021 Mar;82(3):363-370. doi: 10.1016/j.jinf.2020.12.027. 

PMID: 33444699


How artificial intelligence and machine learning can help healthcare systems respond to COVID-19.

van der Schaar M, Alaa AM, Floto A, Gimson A, Scholtes S, Wood A, McKinney E, Jarrett D, Lio P, Ercole A.

Mach Learn. 2020 Dec 9:1-14. doi: 10.1007/s10994-020-05928-x.

PMID: 33318723

Epidemiology of nontuberculous mycobacterial pulmonary disease in Europe and Japan by Delphi estimation.

Schildkraut JA, [...], Floto RA, Hoefsloot W, Griffith DE, Wagner D, Ingen JV

NTM-NET.Respir Med. 2020 Nov;173:106164. doi: 10.1016/j.rmed.2020.106164.

PMID: 32992265

Deletion of cftr Leads to an Excessive Neutrophilic Response and Defective Tissue Repair in a Zebrafish Model of Sterile Inflammation.

Bernut A, Loynes CA, Floto RA, Renshaw SA.

Front Immunol. 2020 Jul 31;11:1733. doi: 10.3389/fimmu.2020.01733. eCollection 2020.

PMID: 32849617

Producing polished prokaryotic pangenomes with the Panaroo pipeline.

Tonkin-Hill G, [...], Floto RA, Frost SDW, Corander J, Bentley SD, Parkhill J.

Genome Biol. 2020 Jul 22;21(1):180. doi: 10.1186/s13059-020-02090-4.

PMID: 32698896 

Cell Surface Remodeling of Mycobacterium abscessus under Cystic Fibrosis Airway Growth Conditions.

Wiersma CJ, [...], Floto RA, Parkhill J, Ordway DJ, Davidson RM, McNeil MR, Jackson M.

ACS Infect Dis. 2020 Aug 14;6(8):2143-2154. doi: 10.1021/acsinfecdis.0c00214. 

PMID: 32551551

How can airborne transmission of COVID-19 indoors be minimised?

Morawska L, Floto A, et al.

Environ Int. 2020 Sep;142:105832. doi: 10.1016/j.envint.2020.105832.

PMID: 32521345 

Peripheral innate immune and bacterial signals relate to clinical heterogeneity in Parkinson's disease.

Wijeyekoon RS, [...], Floto RA, Barker RA, Williams-Gray CH.

Brain Behav Immun. 2020 Jul;87:473-488. doi: 10.1016/j.bbi.2020.01.018. Epub 2020 Jan 30.

PMID: 32006615


***Polymeric nanobiotics as a novel treatment for mycobacterial infections.

Batalha IL, Bernut A, Schiebler M, Ouberai MM, Passemar C, Klapholz C, Kinna S, Michel S, Sader K, Castro-Hartmann P, Renshaw SA, Welland ME, Floto RA.

J Control Release. 2019 Nov 28;314:116-124. doi: 10.1016/j.jconrel.2019.10.009. Epub 2019 Oct 21.

PMID: 31647980 

Relative Contributions of Extracellular and Internalized Bacteria to Early Macrophage Proinflammatory Responses to Streptococcus pneumoniae.

Periselneris J, [...] Floto RA, Noursadeghi M, Brown JS.

mBio. 2019 Sep 24;10(5):e02144-19. doi: 10.1128/mBio.02144-19.

PMID: 31551336 

Same meat, different gravy: ignore the new names of mycobacteria.

Tortoli E, [...], Floto RA, et al.

Eur Respir J. 2019 Jul 11;54(1). pii: 1900795. doi: 10.1183/13993003.00795-2019. 


Development of Inhibitors against Mycobacterium abscessus tRNA (m1G37) Methyltransferase (TrmD) Using Fragment-Based Approaches.

Whitehouse AJ, Thomas SE, Brown KP, Fanourakis A, Chan DS, Libardo MDJ, Mendes V, Boshoff HIM, Floto RA, Abell C, Blundell TL, Coyne AG.

J Med Chem. 2019 Aug 8;62(15):7210-7232. doi: 10.1021/acs.jmedchem.9b00809.


Structure-guided fragment-based drug discovery at the synchrotron: screening binding sites and correlations with hotspot mapping.

Thomas SE, [...], Floto RA, von Delft F, Blundell TL.

Philos Trans A Math Phys Eng Sci. 2019 Jun 17;377(2147):20180422. doi: 10.1098/rsta.2018.0422.


Multi-centre ethics and research governance review can impede non-interventional clinical research.

Duplancic C, Crough T, Bell SC; Australian Non-tuberculous Mycobacteria in Cystic Fibrosis Study Group.

Intern Med J. 2019 Jun;49(6):722-728. doi: 10.1111/imj.14158.


Germline selection shapes human mitochondrial DNA diversity.

Wei W, [...], Floto RA, [...], Chinnery PF.

Science. 2019 May 24;364(6442). pii: eaau6520. doi: 10.1126/science.aau6520.


Targeting TMEM176B Enhances Antitumor Immunity and Augments the Efficacy of Immune Checkpoint Blockers by Unleashing Inflammasome Activation.

Segovia M, [...], Floto RA, [...], Hill M.

Cancer Cell. 2019 May 13;35(5):767-781.e6. doi: 10.1016/j.ccell.2019.04.003.


Advancing Translational Science for Pulmonary Nontuberculous Mycobacterial Infections. A Road Map for Research.

Daniel-Wayman S, [...], Floto RA, et al.

Am J Respir Crit Care Med. 2019 Apr 15;199(8):947-951. doi: 10.1164/rccm.201807-1273PP. No abstract available. 


Pathophysiology, causes and genetics of paediatric and adult bronchiectasis.

Bush A, Floto RA.

Respirology. 2019 Feb 25. doi: 10.1111/resp.13509. [Epub ahead of print] Review.


CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.

Bernut A, Dupont C, Ogryzko NV, Neyret A, Herrmann JL, Floto RA, Renshaw SA, Kremer L.

Cell Rep. 2019 Feb 12;26(7):1828-1840.e4. doi: 10.1016/j.celrep.2019.01.071.


Mabellini: a genome-wide database for understanding the structural proteome and evaluating prospective antimicrobial targets of the emerging pathogen Mycobacterium abscessus.

Skwark MJ, Torres PHM, Copoiu L, Bannerman B, Floto RA, Blundell TL.

Database (Oxford). 2019 Jan 1;2019(1):baz113. doi: 10.1093/database/baz113.

PMID: 31681953 

British Thoracic Society Guideline for bronchiectasis in adults.

Hill AT, Sullivan AL, Chalmers JD, De Soyza A, Elborn SJ, Floto AR, et al.

Thorax. 2019 Jan;74(Suppl 1):1-69. doi: 10.1136/thoraxjnl-2018-212463. No abstract available. 



British Thoracic Society guideline for bronchiectasis in adults.

Hill AT, Sullivan AL, Chalmers JD, De Soyza A, Elborn JS, Floto RA, et al.

BMJ Open Respir Res. 2018 Dec 28;5(1):e000348. doi: 10.1136/bmjresp-2018-000348.


Monocyte Function in Parkinson's Disease and the Impact of Autologous Serum on Phagocytosis.

Wijeyekoon RS, Kronenberg-Versteeg D, Scott KM, Hayat S, Jones JL, Clatworthy MR, Floto RA, Barker RA, Williams-Gray CH.

Front Neurol. 2018 Oct 16;9:870. doi: 10.3389/fneur.2018.00870. eCollection 2018.


Mutations in the MAB_2299c TetR Regulator Confer Cross-Resistance to Clofazimine and Bedaquiline in Mycobacterium abscessus.

Richard M, Gutiérrez AV, Viljoen A, Rodriguez-Rincon D, Roquet-Baneres F, Blaise M, Everall I, Parkhill J, Floto RA, Kremer L.

Antimicrob Agents Chemother. 2018 Dec 21;63(1). pii: e01316-18. doi: 10.1128/AAC.01316-18.


Latent class analysis to define radiological subgroups in pulmonary nontuberculous mycobacterial disease.

Cowman SA, Jacob J, Obaidee S, Andres Floto R, Wilson R, Haworth CS, Loebinger MR.

BMC Pulm Med. 2018 Aug 31;18(1):145. doi: 10.1186/s12890-018-0675-8.



British Thoracic Society Guideline for the management of non-tuberculous mycobacterial pulmonary disease (NTM-PD).

Haworth CS, [...], Floto RA.

BMJ Open Respir Res. 2017 Oct 19;4(1):e000242. doi: 10.1136/bmjresp-2017-000242.


Introducing the new BTS Guideline: Management of non-tuberculous mycobacterial pulmonary disease (NTM-PD).

Haworth CS, Floto RA.

Thorax. 2017 Nov;72(11):969-970. doi: 10.1136/thoraxjnl-2017-210929.


British Thoracic Society guidelines for the management of non-tuberculous mycobacterial pulmonary disease (NTM-PD).

Haworth CS, [...], Floto RA.

Thorax. 2017 Nov;72(Suppl 2):ii1-ii64. doi: 10.1136/thoraxjnl-2017-210927. 


***Genomic epidemiology of a national outbreak of post-surgical Mycobacterium abscessus wound infections in Brazil.

Everall I, [...], Floto RA, Parkhill J, Leão SC, Harris SR.

Microb Genom. 2017 May 3;3(5):e000111.


Examining the Effect of Residual Amikacin on Sputum Culture for Nontuberculous Mycobacteria.

Eagle G, Brown K, Floto RA.

Am J Respir Crit Care Med. 2017 Jun 23. doi: 10.1164/rccm.201704-0792LE.

PMID: 28644036

Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection.

Péan CB, [...], Andres Floto R, Dionne MS.

Nat Commun. 2017 Mar 6;8:14642. 

PMID: 28262681

Poor adherence to management guidelines in nontuberculous mycobacterial pulmonary diseases.

van Ingen J,[..], Floto RA, et al..

Eur Respir J. 2017 Feb 15;49(2).

PMID: 28182571


***Emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium.

Bryant JM*, Grogono DM*,[...], Floto RA.

Science. 2016 : 751-757. 


C13orf31 (FAMIN) is a central regulator of immunometabolic function.

Cader MZ, [...], Floto RA, et al.,.

Nat Immunol. 2016 Aug 1. doi: 10.1038/ni.3532.

PMID: 27478939

Epidemiology of nontuberculous mycobacteria (NTM) amongst individuals with cystic fibrosis (CF).

Viviani L, Harrison MJ, Zolin A, Haworth CS, Floto RA.

J Cyst Fibros. 2016 Mar 31. 

PMID: 27050794


Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Klionsky DJ,[...], Floto RA, et al.,. 

Autophagy. 2016 Jan 2;12(1):1-222. 

PMID: 26799652


US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis: executive summary.

Floto RA, et al.,.

Thorax. 2016 Jan;71(1):88-90. doi: 10.1136/thoraxjnl-2015-207983.

PMID: 26678435


US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis.

Floto RA, et al.,.

Thorax. 2016 Jan;71 Suppl 1:i1-i22. doi: 10.1136/thoraxjnl-2015-207360.

PMID: 26666259



Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

Panagea T, [...], Floto RA, Gilligan P.

J Clin Microbiol. 2015 Jul;53(7):2355-8. doi: 10.1128/JCM.00494-15.

PMID: 25948607


Emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis.

Parkins MD, Floto RA.

J Cyst Fibros. 2015 May;14(3):293-304. doi: 10.1016/j.jcf.2015.03.012. 

PMID: 25881770  Send request


Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors.

Lourenco S, [...], Floto RA, Janes SM.

J Immunol. 2015 Apr 1;194(7):3463-74. doi: 10.4049/jimmunol.1402097. 

PMID: 25712213


The growing threat of nontuberculous mycobacteria in CF.

Floto RA, Haworth CS.

J Cyst Fibros. 2015 Jan;14(1):1-2. doi: 10.1016/j.jcf.2014.12.002.  

PMID: 25487786  Send request



*** Functional drug screening reveals anticonvulsants as enhancers of mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion.

Schiebler M, [...], Floto RA.

EMBO Mol Med. 2014 Dec 22;7(2):127-39. doi: 10.15252/emmm.201404137.

PMID: 25535254


*** Innate Immunity. A Spaetzle-like role for nerve growth factor β in vertebrate immunity to Staphylococcus aureus.

Hepburn L, [...], Floto RA.

Science. 2014 Oct 31;346(6209):641-6. doi: 10.1126/science.1258705.

PMID: 25359976


Autologous dendritic cells prolong allograft survival through Tmem176b-dependent antigen cross-presentation.

Segovia M, [...] Floto RA, et al.,.

Am J Transplant. 2014 May;14(5):1021-31. doi: 10.1111/ajt.12708.

PMID: 24731243


Human urinary exosomes as innate immune effectors.

Hiemstra TF, [...], Floto RA et al.,.

J Am Soc Nephrol. 2014 Sep;25(9):2017-27. doi: 10.1681/ASN.2013101066. 

PMID: 24700864



*** Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study.

Bryant JM, [...], Floto RA.

Lancet. 2013 May 4;381(9877):1551-60. doi: 10.1016/S0140-6736(13)60632-7.

PMID: 23541540


Genomic pathology of SLE-associated copy-number variation at the FCGR2C/FCGR3B/FCGR2B locus.

Mueller M, [...], Floto RA, et al.,.

Am J Hum Genet. 2013 Jan 10;92(1):28-40. doi: 10.1016/j.ajhg.2012.11.013.

PMID: 23261299



Treatment of Mycobacterium abscessus: all macrolides are equal, but perhaps some are more equal than others.

Stout JE, Floto RA.

Am J Respir Crit Care Med. 2012 Nov 1;186(9):822-3. doi: 10.1164/rccm.201208-1500ED.  

PMID: 23118083


Non-tuberculous mycobacteria in cystic fibrosis.

Hill UG, Floto RA, Haworth CS.

J R Soc Med. 2012 Jun;105 Suppl 2:S14-8. doi: 10.1258/jrsm.2012.12s003.  

PMID: 22688361


Constitutive expression of a Mg2+-inhibited K+ current and a TRPM7-like current in human erythroleukemia cells.

Mason MJ, Schaffner C, Floto RA, Teo QA.

Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C853-67. doi: 10.1152/ajpcell.00071.2011.

PMID: 22135214



*** Azithromycin blocks autophagy and may predispose cystic fibrosis patients to mycobacterial infection.

Renna M, [...], Floto RA.

J Clin Invest. 2011 Sep;121(9):3554-63. doi: 10.1172/JCI46095.

PMID: 21804191


Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.

Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC.

J Cell Sci. 2011 Feb 1;124(Pt 3):469-82. doi: 10.1242/jcs.076489.

PMID: 21242315



Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa.

Dai X, [...], Floto RA, et al.,.

Blood. 2009 Jul 9;114(2):318-27. doi: 10.1182/blood-2008-10-184457. 

PMID: 19420354


Rapamycin and mTOR-independent autophagy inducers ameliorate toxicity of polyglutamine-expanded huntingtin and related proteinopathies.

Sarkar S, Ravikumar B, Floto RA, Rubinsztein DC.

Cell Death Differ. 2009 Jan;16(1):46-56. doi: 10.1038/cdd.2008.110.

PMID: 18636076



Novel targets for Huntington's disease in an mTOR-independent autophagy pathway.

Williams A, [...], Floto RA, Rubinsztein DC.

Nat Chem Biol. 2008 May;4(5):295-305. doi: 10.1038/nchembio.79.

PMID: 18391949


Distinct cell-specific control of autoimmunity and infection by FcgammaRIIb.

Brownlie RJ, [...], Floto RA, et al.,.

J Exp Med. 2008 Apr 14;205(4):883-95. doi: 10.1084/jem.20072565.




*** Small molecule enhancers of rapamycin-induced TOR inhibition promote autophagy, reduce toxicity in Huntington's disease models and enhance killing of mycobacteria by macrophages.

Floto RA, Sarkar S, Perlstein EO, Kampmann B, Schreiber SL, Rubinsztein DC.

Autophagy. 2007 Nov-Dec;3(6):620-2. 

PMID: 17786022  Send request


IFN-gamma- and TNF-independent vitamin D-inducible human suppression of mycobacteria: the role of cathelicidin LL-37.

Martineau AR, [...], Floto RA, et al.,.

J Immunol. 2007 Jun 1;178(11):7190-8.

PMID: 17513768


Systemic lupus erythematosus-associated defects in the inhibitory receptor FcgammaRIIb reduce susceptibility to malaria.

Clatworthy MR, [...], Floto RA, Smith KG.

Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7169-74. 

PMID: 17435165



*** Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5.

Floto RA, [...], Smith KG, Lehner PJ.

Science. 2006 Oct 20;314(5798):454-8.

PMID: 17053144  Send request



Lithium induces autophagy by inhibiting inositol monophosphatase.

Sarkar S, Floto RAet al.,.

J Cell Biol. 2005 Sep 26;170(7):1101-11.

PMID: 16186256


Loss of function of a lupus-associated FcgammaRIIb polymorphism through exclusion from lipid rafts.

Floto RAet al.,.

Nat Med. 2005 Oct;11(10):1056-8.

PMID: 16170323  Send request



HSP70 peptide binding mutants separate antigen delivery from dendritic cell stimulation.

MacAry PA, Javid B, Floto RA, Smith KG, Oehlmann W, Singh M, Lehner PJ.

Immunity. 2004 Jan;20(1):95-106.

PMID: 14738768



The vagus nerve, macrophages, and nicotine.

Floto RA, Smith KG.

Lancet. 2003 Mar 29;361(9363):1069-70. 

PMID: 12672307




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