Major Publications
2025
***Metabolic control of porin permeability influences antibiotic resistance in Escherichia coli.
Caño Muñiz SE, Trigg S, Hardo G, Hagting A, Evans IE, Ruis C, Alsulami AF, Summers D, Crawshay-Williams F, Blundell TL, Boeck L, Bakshi S, Floto RA.
Nat Microbiol. 2025 Dec;10(12):3202-3214. doi: 10.1038/s41564-025-02175-5. Epub 2025 Nov 24.
***Phagosomal RNA sensing through TLR8 controls susceptibility to tuberculosis.
Maserumule C*, Passemar C*, Oh OSH, Hegyi K, Brown K, Weimann A, Dinan A, Davila S, Klapholz C, Bryant J, Verma D, Gadwa J, Krishnananthasivam S, Vongtongsalee K, Kendall E, Trelles A, Hibberd ML, Sanz J, Bertol J, Vázquez-Iniesta L, Andi K, Kumar SS, Ordway D, Prados-Rosales R, MacAry PA, Floto RA.
Cell Rep. 2025 May 27;44(5):115657. doi: 10.1016/j.celrep.2025.115657. Epub 2025 May 6.
Mutations in mexT bypass the stringent response dependency of virulence in Pseudomonas aeruginosa.
Figueroa W, Cazares A, Ashworth EA, Weimann A, Kadioglu A, Floto RA, Welch M.
Cell Rep. 2025 Jan 28;44(1):115079. doi: 10.1016/j.celrep.2024.115079. Epub 2024 Dec 20.
Microbial metabolism disrupts cytokine activity to impact host immune response.
Marshall EKP, Nunes C, Burbaud S, Vincent CM, Munroe NO, Simoes da Silva CJ, Wadhawan A, Pearson WH, Sangen J, Boeck L, Floto RA, S Dionne M.
Proc Natl Acad Sci U S A. 2024 Nov 12;121(46):e2405719121. doi: 10.1073/pnas.2405719121
Global genomic surveillance of monkeypox virus.
Otieno JR, Ruis C, [...], Floto RA, Cunningham J, Van Kerkhove MD, Lewis RF, Subissi L.
Nat Med. 2025 Jan;31(1):342-350. doi: 10.1038/s41591-024-03370-3.
2024
***Evolution and host-specific adaptation of Pseudomonas aeruginosa.
Weimann A, Dinan AM, Ruis C, Bernut A, Pont S, Brown K, Ryan J, Santos L, Ellison L, Ukor E, Pandurangan AP, Krokowski S, Blundell TL, Welch M, Blane B, Judge K, Bousfield R, Brown N, Bryant JM, Kukavica-Ibrulj I, Rampioni G, Leoni L, Harrison PT, Peacock SJ, Thomson NR, Gauthier J, Fothergill JL, Levesque RC, Parkhill J, Floto RA.
Science. 2024 Jul 5;385(6704):eadi0908. doi: 10.1126/science.adi0908.
Extracellular vesicles in mycobacteria: new findings in biogenesis, host-pathogen interactions, and diagnostics.
Salgueiro VC, Passemar C, Vázquez-Iniesta L, Lerma L, Floto A, Prados-Rosales R.
mBio. 2024 May 8;15(5):e0255223. doi: 10.1128/mbio.02552-23.
In vitro platform to model the function of ionocytes in the human airway epithelium.
Vilà-González M, Pinte L, Fradique R, [...], Floto RA, Rawlins EL, Cicuta P, Vallier L.
Respir Res. 2024 Apr 25;25(1):180. doi: 10.1186/s12931-024-02800-7.
Lipoarabinomannan modification as a source of phenotypic heterogeneity in host-adapted Mycobacterium abscessus isolates.
De K, Belardinelli JM, Pandurangan AP, Ehianeta T, Lian E, Palčeková Z, Lam H, Gonzalez-Juarrero M, Bryant JM, Blundell TL, Parkhill J, Floto RA, Lowary TL, Wheat WH, Jackson M.
Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2403206121. doi: 10.1073/pnas.2403206121. Epub 2024 Apr 17.
2023
***Mutational spectra are associated with bacterial niche.
Ruis C, Weimann A, Tonkin-Hill G, Pandurangan AP, Matuszewska M, Murray GGR, Lévesque RC, Blundell TL, Floto RA, Parkhill J.
Nat Commun. 2023 Nov 4;14(1):7091. doi: 10.1038/s41467-023-42916-w.
Clinically relevant mutations in the PhoR sensor kinase of host-adapted Mycobacterium abscessus isolates impact response to acidic pH and virulence.
Belardinelli JM, Arora D, Avanzi C, Wheat WH, Bryant JM, Spencer JS, Blundell TL, Parkhill J, Floto RA, Jackson M.
Microbiol Spectr. 2023 Dec 12;11(6):e0158823. doi: 10.1128/spectrum.01588-23.
***A lung-specific mutational signature enables inference of viral and bacterial respiratory niche.
Ruis C, Peacock TP, Polo LM, Masone D, Alvarez MS, Hinrichs AS, Turakhia Y, Cheng Y, McBroome J, Corbett-Detig R, Parkhill J, Floto RA.
Microb Genom. 2023 May;9(5):mgen001018. doi: 10.1099/mgen.0.001018.
2022
Structural Characterization of Mycobacterium abscessus Phosphopantetheine Adenylyl Transferase Ligand Interactions: Implications for Fragment-Based Drug Design.
Thomas SE, McCarthy WJ, El Bakali J, Brown KP, Kim SY, Blaszczyk M, Mendes V, Abell C, Floto RA, Coyne AG, Blundell TL.
Front Mol Biosci. 2022 May 30;9:880432. doi: 10.3389/fmolb.2022.880432.
Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses.
Meng JX, Zhang Y, Saman D, Haider AM, De S, Sang JC, Brown K, Jiang K, Humphrey J, Julian L, Hidari E, Lee SF, Balmus G, Floto RA, Bryant CE, Benesch JLP, Ye Y, Klenerman D.
Nat Commun. 2022 May 16;13(1):2692. doi: 10.1038/s41467-022-30461-x.
Using Structure-guided Fragment-Based Drug Discovery to Target Pseudomonas aeruginosa Infections in Cystic Fibrosis.
Arif SM, Floto RA, Blundell TL.
Front Mol Biosci. 2022 Mar 30;9:857000. doi: 10.3389/fmolb.2022.857000.
***Antibiotic Management in Bronchiectasis.
Haworth CS, Floto RA.
Clin Chest Med. 2022 Mar;43(1):165-177. doi: 10.1016/j.ccm.2021.11.009.
Development of Inhibitors of SAICAR Synthetase (PurC) from Mycobacterium abscessus Using a Fragment-Based Approach.
Charoensutthivarakul S, Thomas SE, Curran A, Brown KP, [...], Floto RA, Blundell TL, Coyne AG, Abell C.
ACS Infect Dis. 2022 Feb 11;8(2):296-309. doi: 10.1021/acsinfecdis.1c00432.
PMID: 35037462
2021
100,000 Genomes Pilot on Rare-Disease Diagnosis in Health Care - Preliminary Report.
100,000 Genomes Project Pilot Investigators,[...], Floto RA, et al.
N Engl J Med. 2021 Nov 11;385(20):1868-1880.
PMID: 34758253
***Dissemination of Mycobacterium abscessus via global transmission networks.
Ruis C, Bryant JM, Bell SC, Thomson R, Davidson RM, Hasan NA, van Ingen J, Strong M, Floto RA, Parkhill J.
Nat Microbiol. 2021 Oct;6(10):1279-1288.
***Integrated human/SARS-CoV-2 metabolic models present novel treatment strategies against COVID-19.
Bannerman BP, Júlvez J, Oarga A, Blundell TL, Moreno P, Floto RA.
Life Sci Alliance. 2021 Aug 5;4(10):e202000954.
*** Stepwise pathogenic evolution of Mycobacterium abscessus.
Bryant JM, Brown KP, Burbaud S, Everall I, Belardinelli JM, Rodriguez-Rincon D, Grogono DM, Peterson CM, Verma D, Evans IE, Ruis C, Weimann A, Arora D, Malhotra S, Bannerman B, Passemar C, Templeton K, MacGregor G, Jiwa K, Fisher AJ, Blundell TL, Ordway DJ, Jackson M, Parkhill J, Floto RA.
Science. 2021 Apr 30;372(6541):eabb8699. doi: 10.1126/science.abb8699.
The Impact of Hospital-Ward Ventilation on Airborne-Pathogen Exposure.
Mingotti N, Grogono D, [...], Floto RA, Woods AW.
Am J Respir Crit Care Med. 2021 Mar 15;203(6):766-769. doi: 10.1164/rccm.202009-3634LE.
PMID: 33211973
2020
Producing polished prokaryotic pangenomes with the Panaroo pipeline.
Tonkin-Hill G, [...], Floto RA, Frost SDW, Corander J, Bentley SD, Parkhill J.
Genome Biol. 2020 Jul 22;21(1):180. doi: 10.1186/s13059-020-02090-4.
Wijeyekoon RS, [...], Floto RA, Barker RA, Williams-Gray CH.
Brain Behav Immun. 2020 Jul;87:473-488. doi: 10.1016/j.bbi.2020.01.018. Epub 2020 Jan 30.
PMID: 32006615
2019
***Polymeric nanobiotics as a novel treatment for mycobacterial infections.
Batalha IL, Bernut A, Schiebler M, Ouberai MM, Passemar C, Klapholz C, Kinna S, Michel S, Sader K, Castro-Hartmann P, Renshaw SA, Welland ME, Floto RA.
J Control Release. 2019 Nov 28;314:116-124. doi: 10.1016/j.jconrel.2019.10.009. Epub 2019 Oct 21.
PMID: 31647980
Structure-guided fragment-based drug discovery at the synchrotron: screening binding sites and correlations with hotspot mapping.
Thomas SE, [...], Floto RA, von Delft F, Blundell TL.
Philos Trans A Math Phys Eng Sci. 2019 Jun 17;377(2147):20180422. doi: 10.1098/rsta.2018.0422.
Germline selection shapes human mitochondrial DNA diversity.
Wei W, [...], Floto RA, [...], Chinnery PF.
Science. 2019 May 24;364(6442). pii: eaau6520. doi: 10.1126/science.aau6520.
PMID:31123110
Pathophysiology, causes and genetics of paediatric and adult bronchiectasis.
Bush A, Floto RA.
Respirology. 2019 Feb 25. doi: 10.1111/resp.13509. [Epub ahead of print] Review.
PMID:30801930
CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
Bernut A, Dupont C, Ogryzko NV, Neyret A, Herrmann JL, Floto RA, Renshaw SA, Kremer L.
Cell Rep. 2019 Feb 12;26(7):1828-1840.e4. doi: 10.1016/j.celrep.2019.01.071.
PMID:30759393
Skwark MJ, Torres PHM, Copoiu L, Bannerman B, Floto RA, Blundell TL.
Database (Oxford). 2019 Jan 1;2019(1):baz113. doi: 10.1093/database/baz113.
British Thoracic Society Guideline for bronchiectasis in adults.
Hill AT, Sullivan AL, Chalmers JD, De Soyza A, Elborn SJ, Floto AR, et al.
Thorax. 2019 Jan;74(Suppl 1):1-69. doi: 10.1136/thoraxjnl-2018-212463. No abstract available.
PMID:30545985
2018
British Thoracic Society guideline for bronchiectasis in adults.
Hill AT, Sullivan AL, Chalmers JD, De Soyza A, Elborn JS, Floto RA, et al.
BMJ Open Respir Res. 2018 Dec 28;5(1):e000348. doi: 10.1136/bmjresp-2018-000348.
Mutations in the MAB_2299c TetR Regulator Confer Cross-Resistance to Clofazimine and Bedaquiline in Mycobacterium abscessus.
Richard M, Gutiérrez AV, Viljoen A, Rodriguez-Rincon D, Roquet-Baneres F, Blaise M, Everall I, Parkhill J, Floto RA, Kremer L.
Antimicrob Agents Chemother. 2018 Dec 21;63(1). pii: e01316-18. doi: 10.1128/AAC.01316-18.
2017
British Thoracic Society Guideline for the management of non-tuberculous mycobacterial pulmonary disease (NTM-PD).
Haworth CS, [...], Floto RA.
BMJ Open Respir Res. 2017 Oct 19;4(1):e000242. doi: 10.1136/bmjresp-2017-000242.
PMID:29449949
Introducing the new BTS Guideline: Management of non-tuberculous mycobacterial pulmonary disease (NTM-PD).
Haworth CS, Floto RA.
Thorax. 2017 Nov;72(11):969-970. doi: 10.1136/thoraxjnl-2017-210929.
PMID:29054887
British Thoracic Society guidelines for the management of non-tuberculous mycobacterial pulmonary disease (NTM-PD).
Haworth CS, [...], Floto RA.
Thorax. 2017 Nov;72(Suppl 2):ii1-ii64. doi: 10.1136/thoraxjnl-2017-210927.
***Genomic epidemiology of a national outbreak of post-surgical Mycobacterium abscessus wound infections in Brazil.
Everall I, [...], Floto RA, Parkhill J, Leão SC, Harris SR.
Microb Genom. 2017 May 3;3(5):e000111.
Examining the Effect of Residual Amikacin on Sputum Culture for Nontuberculous Mycobacteria.
Eagle G, Brown K, Floto RA.
Am J Respir Crit Care Med. 2017 Jun 23. doi: 10.1164/rccm.201704-0792LE.
Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection.
Péan CB, [...], Andres Floto R, Dionne MS.
Nat Commun. 2017 Mar 6;8:14642.
2016
***Emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium.
Bryant JM*, Grogono DM*,[...], Floto RA.
Science. 2016 : 751-757.
C13orf31 (FAMIN) is a central regulator of immunometabolic function.
Cader MZ, [...], Floto RA, et al.,.
Nat Immunol. 2016 Aug 1. doi: 10.1038/ni.3532.
US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis: executive summary.
Floto RA, et al.,.
Thorax. 2016 Jan;71(1):88-90. doi: 10.1136/thoraxjnl-2015-207983.
US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis.
Floto RA, et al.,.
Thorax. 2016 Jan;71 Suppl 1:i1-i22. doi: 10.1136/thoraxjnl-2015-207360.
2015
Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
Panagea T, [...], Floto RA, Gilligan P.
J Clin Microbiol. 2015 Jul;53(7):2355-8. doi: 10.1128/JCM.00494-15.
Emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis.
Parkins MD, Floto RA.
J Cyst Fibros. 2015 May;14(3):293-304. doi: 10.1016/j.jcf.2015.03.012.
Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors.
Lourenco S, [...], Floto RA, Janes SM.
J Immunol. 2015 Apr 1;194(7):3463-74. doi: 10.4049/jimmunol.1402097.
The growing threat of nontuberculous mycobacteria in CF.
Floto RA, Haworth CS.
J Cyst Fibros. 2015 Jan;14(1):1-2. doi: 10.1016/j.jcf.2014.12.002.
2014
*** Functional drug screening reveals anticonvulsants as enhancers of mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion.
Schiebler M, [...], Floto RA.
EMBO Mol Med. 2014 Dec 22;7(2):127-39. doi: 10.15252/emmm.201404137.
*** Innate Immunity. A Spaetzle-like role for nerve growth factor β in vertebrate immunity to Staphylococcus aureus.
Hepburn L, [...], Floto RA.
Science. 2014 Oct 31;346(6209):641-6. doi: 10.1126/science.1258705.
2013
*** Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study.
Bryant JM, [...], Floto RA.
Lancet. 2013 May 4;381(9877):1551-60. doi: 10.1016/S0140-6736(13)60632-7.
Genomic pathology of SLE-associated copy-number variation at the FCGR2C/FCGR3B/FCGR2B locus.
Mueller M, [...], Floto RA, et al.,.
Am J Hum Genet. 2013 Jan 10;92(1):28-40. doi: 10.1016/j.ajhg.2012.11.013.
2012
Treatment of Mycobacterium abscessus: all macrolides are equal, but perhaps some are more equal than others.
Stout JE, Floto RA.
Am J Respir Crit Care Med. 2012 Nov 1;186(9):822-3. doi: 10.1164/rccm.201208-1500ED.
Non-tuberculous mycobacteria in cystic fibrosis.
Hill UG, Floto RA, Haworth CS.
J R Soc Med. 2012 Jun;105 Suppl 2:S14-8. doi: 10.1258/jrsm.2012.12s003.
Constitutive expression of a Mg2+-inhibited K+ current and a TRPM7-like current in human erythroleukemia cells.
Mason MJ, Schaffner C, Floto RA, Teo QA.
Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C853-67. doi: 10.1152/ajpcell.00071.2011.
2011
*** Azithromycin blocks autophagy and may predispose cystic fibrosis patients to mycobacterial infection.
Renna M, [...], Floto RA.
J Clin Invest. 2011 Sep;121(9):3554-63. doi: 10.1172/JCI46095.
Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC.
J Cell Sci. 2011 Feb 1;124(Pt 3):469-82. doi: 10.1242/jcs.076489.
2009
Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa.
Dai X, [...], Floto RA, et al.,.
Blood. 2009 Jul 9;114(2):318-27. doi: 10.1182/blood-2008-10-184457.
Rapamycin and mTOR-independent autophagy inducers ameliorate toxicity of polyglutamine-expanded huntingtin and related proteinopathies.
Sarkar S, Ravikumar B, Floto RA, Rubinsztein DC.
Cell Death Differ. 2009 Jan;16(1):46-56. doi: 10.1038/cdd.2008.110.
2008
Novel targets for Huntington's disease in an mTOR-independent autophagy pathway.
Williams A, [...], Floto RA, Rubinsztein DC.
Nat Chem Biol. 2008 May;4(5):295-305. doi: 10.1038/nchembio.79.
Distinct cell-specific control of autoimmunity and infection by FcgammaRIIb.
Brownlie RJ, [...], Floto RA, et al.,.
J Exp Med. 2008 Apr 14;205(4):883-95. doi: 10.1084/jem.20072565.
2007
*** Small molecule enhancers of rapamycin-induced TOR inhibition promote autophagy, reduce toxicity in Huntington's disease models and enhance killing of mycobacteria by macrophages.
Floto RA, Sarkar S, Perlstein EO, Kampmann B, Schreiber SL, Rubinsztein DC.
Autophagy. 2007 Nov-Dec;3(6):620-2.
IFN-gamma- and TNF-independent vitamin D-inducible human suppression of mycobacteria: the role of cathelicidin LL-37.
Martineau AR, [...], Floto RA, et al.,.
J Immunol. 2007 Jun 1;178(11):7190-8.
Systemic lupus erythematosus-associated defects in the inhibitory receptor FcgammaRIIb reduce susceptibility to malaria.
Clatworthy MR, [...], Floto RA, Smith KG.
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7169-74.
2006
*** Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5.
Floto RA, [...], Smith KG, Lehner PJ.
Science. 2006 Oct 20;314(5798):454-8.
2005
Lithium induces autophagy by inhibiting inositol monophosphatase.
Sarkar S, Floto RA, et al.,.
J Cell Biol. 2005 Sep 26;170(7):1101-11.
Loss of function of a lupus-associated FcgammaRIIb polymorphism through exclusion from lipid rafts.
Floto RA, et al.,.
Nat Med. 2005 Oct;11(10):1056-8.
2004
HSP70 peptide binding mutants separate antigen delivery from dendritic cell stimulation.
MacAry PA, Javid B, Floto RA, Smith KG, Oehlmann W, Singh M, Lehner PJ.
Immunity. 2004 Jan;20(1):95-106.
2003
The vagus nerve, macrophages, and nicotine.
Floto RA, Smith KG.
Lancet. 2003 Mar 29;361(9363):1069-70.